Sue Sylvester is a fictional character from the Fox (Fox Broadcasting Company) musical (musical film) comedy-drama series Glee (Glee (TV series)). The character is portrayed by actress Jane Lynch, and has appeared in Glee from its pilot episode (Pilot (Glee)), first broadcast on May 19, 2009. Sue was developed by Glee creators Ryan Murphy (Ryan Murphy (writer)), Brad Falchuk and Ian Brennan (Ian Brennan (writer)). She was originally intended to be a recurring character, but was promoted to a starring role when a Damon Wayans pilot (pilot episode) Lynch was working on for ABC (ABC (network)) fell through.

Lynch has characterized Sue as willing to do "whatever it takes" to win, noting that although the character does have a heart, her softer side will not often be seen in the show. Her storylines have seen her continuously conspire against William McKinley High Schools glee club, irritated that their increasing success comes at the detriment of her own cheerleading budget. After giving the clubs set-list for Sectionals to rival glee directors in an attempt at sabotage, Sue is suspended from the school, though blackmails her way back.

The character has been generally well received by critics. Mary McNamara for the Los Angeles Times has written that "Lynch alone makes Glee worth watching", while Entertainment Weeklys Ken Tucker has called Sue "the greatest Broadway (Broadway theatre)-musical villain to ever co-star in a TV series". On December 15, 2009, Lynch was nominated for the Best Supporting Actress - Series, Miniseries or Television Film (Golden Globe Award for Best Supporting Actress - Series, Miniseries or Television Film) Golden Globe Award for her performance in the role.